Enhanced carbohydrate structural selectivity in ion mobility-mass spectrometry analyses by boronic acid derivatization.
نویسندگان
چکیده
The boronic acid derivatization of carbohydrates is demonstrated as an ion mobility shift strategy to improve confidence in the identification and characterization of carbohydrate assignments using ion mobility-mass spectrometry.
منابع مشابه
Structural resolution of carbohydrate positional and structural isomers based on gas-phase ion mobility-mass spectrometry.
This report describes the rapid characterization of positional and structural carbohydrate isomers based on structural separations provided by ion mobility-mass spectrometry (IM-MS). Many of the diseases associated with glycoprotein variation can be more effectively treated with earlier detection substantiating the need for high-throughput methodologies for glycan characterization. This remains...
متن کاملElectron-capturing derivatization of neutral steroids for increasing sensitivity in liquid chromatography/negative atmospheric pressure chemical ionization-mass spectrometry.
Derivatization of neutral steroids for increasing sensitivity in liquid chromatography/negative atmospheric pressure chemical ionization-mass spectrometry (APCI-MS) has been examined. Under APCI conditions, gas-phase electrons are provided by the corona discharge and captured by electron-affinitive compounds. In negative APCI-MS, therefore, ultrahigh sensitivity can be obtained by tagging neutr...
متن کاملCarbohydrate and Glycoconjugate Analysis by Ion Mobility Mass Spectrometry: Opportunities and Challenges
Carbohydrates are characterized by a truly remarkable level of structural diversity which stands in accord with a corresponding functional diversity arguably unsurpassed by any other class of biomolecule. As metabolites, structural components, mediators of molecular interaction, and modifiers of other biomolecules, carbohydrates play central roles in numerous areas of biochemistry. Detailed mol...
متن کاملIdentification of structurally closely related monosaccharide and disaccharide isomers by PMP labeling in conjunction with IM-MS/MS
It remains particularly difficult for gaining unambiguous information on anomer, linkage, and position isomers of oligosaccharides using conventional mass spectrometry (MS) methods. In our laboratory, an ion mobility (IM) shift strategy was employed to improve confidence in the identification of structurally closely related disaccharide and monosaccharide isomers using IMMS. Higher separation b...
متن کاملLiquid Chromatography Tandem Mass/Mass Spectrometry for the Quantification of Fudosteine in Human Serum without Precolumn Derivatization
A quantitative analysis method for fudosteine in human serum by high-performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI/MS/MS) was established, which shows high sensitivity and selectivity. The mobile phase composition was 75% 20 mM acetic acid and 25% acetonitril, which was pumped at a flow rate of 0.40 mL/min. The overall chromatographic run time was ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Chemical communications
دوره 43 شماره
صفحات -
تاریخ انتشار 2008